The Wizard guides you during the development of the method
OSIRIS speeds up the development of your HPLC separations in isocratic mode as well as for gradient operation. Usually, the set-up of a complete method requires time-consuming analysis in your laboratory before obtaining a satisfactory result. With a minimum of experimentation OSIRIS is able to model retention behaviour of compounds and then to offer computer predictions of various chromatograms for different analytical conditions. Using this predictive modelling, a wide range of conditions can be simulated in only few minutes.
The chromatographic behavior of the solutes is modelled as a function of the elution conditions. This model is obtained from the results and the chromatographic data of a few preliminary runs. OSIRIS guides the user by proposing conditions for these preliminary runs. The number of runs and the conditions depends on the type of optimization process :
- Mobile phase composition (isocratic, binary, ternary or quaternary, linear and multi-step gradient)
- Mobile phase/pH.
- Mobile phase/Temperature