-Separation conditions set up
-HPLC method development
-Concept of robustness
-Optimization of 1 parameter (composition, pH or temperature)
-Simultaneous optimization of 2 or 3 parameters
-Description of Osiris
-Illustration of the use of OSIRIS (optimisation of 1 parameter, of 2 or 3 parameters simultaneously, linear and multi-linear gradient slope and composition)